Study of the Effect of Bacteriocin-producing Bacillus subtilis Strains on Beta-lactamase-producing Pathogenic Bacteria

Background: The emergence of antimicrobial resistance results in morbidities and mortalities associated with many bacterial infections is a major concerning issue front the healthcare system. As part competitive adaptive strategy for its growth, gram-positive bacteria like Bacillus subtilis produce peptides; bacteriocins. These peptides protect other species. Objectives: To counter resistance, bacteriocins can be considered potential drug option against drug-resistant microorganisms. Methods: After isolation characterisation B. from 5 collected soil samples, bacteriocin was extracted these by using ‘solvent extraction method’, characterised, then purified to evaluate activity pathogenic like; Streptococcus pyogenes, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae Acinetobacter baumannii ‘Kirby-Bauer disc diffusion’, method. stability also investigated, at different temperatures, pH incubation times. Results: showed great S. followed typhi P. aeruginosa. However, lowest A. baumannii. With increase time temperature, decreased, indicating protein nature bacteriocin. Compared standard antibiotics, isolated demonstrated significant inhibitory effect tested pathogens. Conclusions: From this study, it concluded that become alternative available medicines. They used antimicrobial-resistant because their pathogens compared antibiotics.